Biotechnology | University of Parma

Professor: Elena MAESTRI
Academic discipline: Biologia applicata (BIO/13)
Field: Discipline biotecnologiche con finalità specifiche: biologiche e industriali
Type of training activity: Characterising

52 hours
of face-to-face activities

6 credits

hub: PARMA

course unit
in ITALIAN

lectures timetable

exam calls

Integrated course unit module: RECOMBINANT TECHNOLOGIES AND INTEGRATED BIOTECHNOLOGIES LABORATORY II

Learning objectives

The aim of the course is to illustrate the basic methods of recombinant technologies and their applications in plants and animals.

Prerequisites

- - -

Course unit content

The course covers the techniques and approaches for genetic engineering in eukaryotes.

Full programme

Cloning in plants

Cloning vectors for plant organisms – Transformation methods for plant organisms - Obtainment of transgenic plants

Cloning in animal cells and applications in health field

Growth and properties of animal cells in culture - Selection systems for animal cells - Gene transfer into animal cells - Plasmid vectors for animal cells, amplicons and expression vectors - The fate of transferred DNA - Viral vectors and retroviral vectors for animal cells. Gene therapy: principles and methods - Experimental models – Some experimental results - Potential human models

Bibliography

T.A. Brown – Biotecnologie molecolari. Zanichelli
T. Strachan, A. Read – Genetica molecolare umana. Zanichelli
B.R. Glick, J.J. Pasternak, C.L. Patten – Molecular biotechnology. ASM Press
S.B. Primrose, R.M.Twyman – Principles of gene manipulation and genomics. Wiley-Blackwell

Teaching methods

The theoretical classes are integrated with seminars and practical training in laboratory.

Assessment methods and criteria

The oral examination verifies the knowledge acquired by the student and the communication skills. The written exam and the laboratory report verifies knowledge and application of knowledge. In all cases, the learning ability is verified.

Other information